Latest Microbiology Research Month: April-2019
Optimization of Rock Phosphate Solubilization in Submerged Cultures Containing Some Agro-Industrial Residues
Aims: To solubilize rock phosphate in presence of agriculture wastes by microorganisms and manufacture hard answer made with soluble phosphate.
Study Design: The impact of agriculture wastes, rock phosphate concentrations and culture conditions on rock phosphate solubilization. Identification of the potential isolate.
Place and period of Study: biological science Dept., Fac. of Agriculture, personal Shams Univ., Cairo, Egypt, 2015/ 2016.
Methodology: Four isolates were tested on seven agriculture wastes for rock phosphate solubilization. optimisation of some environmental factors. Identification of flora isolate by 18S rRNA sequence analysis.
Results: Some agro-industrial residues (bagasse, corn cobs, black sugar cane syrup, olive cake wastes, rice straw, sugar beet waste and whey) were used as a carbon supply at totally different treatments for highest rock phosphate solubilization (RPS) by bacteria genus sp. Rs7 and Rs22, and Aspergillus sp. Bf6 and RPf10 isolates. the utmost RPS was obtained by the tested bacterium and fungi once each were big on whey and sugar beet waste as a full medium that inflated the fermentation amount to ten and twelve days. All treatments of sugar beet wastes, bagasse, rice straw and corncobs did not support RPS by microorganism isolates. additionally corn cobs showed negative impact on RPS all told treatments for flora isolates. In serial experiments, the utmost RPS was obtained by the tested bacterium and fungi in media supplemented with seven.0 and 10.0 gL-1 RP at pH scale seven.0 and 5.5 inoculated with third-dimensional customary matter and incubated at 30°C severally. The hard answer made by Aspergillus sp. RPf10 containing higher amounts of acid (543.39 μgP ml-1) and indole-3-acetic acid (11.96 mgL-1) also as higher enzyme activity than that made by different tested isolates. This isolate was known as Aspergillus tubingensis RPf10.
Conclusion: the flexibility of Aspergillus tubingensis to convert sugar beet waste and insoluble rock phosphate into hard answer containing most level of soluble phosphate, growth promoting and organic acids which may be apply this answer in future in microbic nutrient and agriculture field. [1]
Isolation and Evaluation of Bacteria Exhibiting Multiple Plant Growth Traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense)
Aim: The study known and evaluated microorganism exhibiting multiple plant growth traits within the Rhizosphere of Yellow Bell Pepper (Capsicum chinense).
Study Design: Seeds of bush chinense were planted during a soil and allowed to grow. when 5 eeks of planting, soil samples from the rhizosphere were collected and also the microorganism community gift within the rhizosphere soil of bush chinense was studied. The isolated organisms were assessed for his or her ability to supply plant growth promoting traits.
Place associate degreed length of Study: This study was administered at an agricultural analysis farmland within the Federal University of Technology, Owerri, Nigeria.
Methodology: Seeds of Capiscum chinense were planted within the soil samples during a greenhouse. Rhizosphere soil was collected for analysis to spot the microorganism composition of the rhizosphere soil.
Results: during this study the presence of true bacteria genus Cereus, staphylococci aureus, true bacteria sp, Enterococcus feacalis and true bacteria polymyxawere evident within the rhizosphere samples collected. All isolates showed multiple plant growth promoting traits except staphylococci aureushich was positive for compound production solely.
Conclusion: The results from this study showed that the microorganism community gift within the soil may be wont to impact important vegetative crop yield and agricultural production. The isolated rhizobacteria may be developed as bio-fertilizers or bioinnoculants, etc. [2]
Comparative Evaluation of Methicillin-resistant Staphylococcus aureus (MRSA) Isolates from Hospital and Community Settings in Nigeria
The ability of staph aureus to resist cefoxitin amongst different antibiotics has created it a major public pathological state in hospital and community settings. during this study, the incidence of cefoxitin (methicillin) resistant S. aureus (MRSA) within the University of Calabar eye and community was evaluated once getting consent and moral approval. a complete of one hundred fifty clinical samples collected from participants seen at the eye and community settings were analyzed. Isolates were known and characterised following customary microbiological procedures whereas antimicrobial sensitivity was administered mistreatment the disc diffusion technique. a complete of forty two S. aureus strains were isolated, out of that 27(64.3%) were from the eye and 15(35.7%) were from the community samples. Antimicrobial status take a look ating of take a look at isolates showed high resistance to the test antibiotics with cefoxitin being the very best (60%). Out of the hr MRSA recovered, 74.1% (20/27) were from University of Calabar eye whereas thirty three.3% (5/15) were from the Community. additionally, MRSA isolates from each locations additionally showed resistance to different antibiotics together with Amoxil, ampicillin+cloxacillin, levofloxacin, norfloxacin and Ethril. This study disclosed a high incidence of Hospital-setting methicillin-resistant S. aureus (HA-MRSA) strains compared to Community-setting methicillin-resistant S. aureus (CA-MRSA) strains. This study any disclosed that MRSA were multi-drug resistant. Thus, sensible infection management practices together with characteristic and treating MRSA carriers, moderate use of antibiotics and hand laundry might scale back the burden related to MRSA-related infections. To any establish and characterize multidrug resistant S. aureus strains, constitution studies could also be used. [3]
Reference
[1] A. Abou-Taleb, K., A. Amin, S., M. Abdelhady, H. and H. Tayeb, Z. (2018) “Optimization of Rock Phosphate Solubilization in Submerged Cultures Containing Some Agro-Industrial Residues”, Journal of Advances in Microbiology, 11(2), pp. 1-18. doi: 10.9734/JAMB/2018/41624.(web link)
[2] Chinakwe, E. C., Nwogwugwu, N. U., Ibekwe, V. I., Chinakwe, P. O., Egbadon, E. O. and Adeleye, S. A. (2019) “Isolation and Evaluation of Bacteria Exhibiting Multiple Plant Growth Traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense)”, Journal of Advances in Microbiology, 14(4), pp. 1-6. doi: 10.9734/jamb/2019/v14i430070.(web link)
[3] N. Mbim, E., I. Mboto, C., O. Edet, U., F. Umego, C., E. George, U., & Temidayo, I. (2017). Comparative Evaluation of Methicillin-resistant Staphylococcus aureus (MRSA) Isolates from Hospital and Community Settings in Nigeria. Journal of Advances in Microbiology, 4(4), 1-9. https://doi.org/10.9734/JAMB/2017/34542.(web link)