Comparative Evaluation of Crude oil Degradability Efficiency of Bacillus amyloliquefaciens and Comam
Comparative evaluation of crude oil degradability efficiency of Bacillus amyloliquefaciens and Comamonas testosteroni with nutrient amendment were investigated in Crude oil Contaminated Soil. The bacteria species used in this study were isolated from the soil collected from Rivers state university research farm using standard microbiological methods. Evaluation and monitoring of bioremediation were done for a period of 56 days while analyses were carried at a constant interval of seven (7) days. Seven (7) experimental set-ups were employed using black polythene bag, The bags were perforated to enhance aeration, each containing 5 kg of agricultural soil and left to fallow for 6 days, on the seventh day each of the experimental set-ups (5kg of soil) except the control (CTRL) were contaminated with crude oil (COCS) giving initial Total Petroleum Hydrocarbon (TPH) value of 10328.03 mg/kg; after which it was allowed for 21 days to ensure even distribution and soil-oil bonding to mimic crude oil spill site before application of augmenting bacteria; Bacillus amyloliquefaciens (BC) and Comamonas testosteroni (CM) and the stimulant; Goat manure (GM). Soil profile before and after contamination were analyzed while parameters like Sulphate, pH, Nitrate and Total Petroleum Hydrocarbon (TPH), as well as microbial analyses such as Total Heterotrophic Bacteria (THB), Total Heterotrophic Fungi (THF), Hydrocarbon Utilizing Bacteria (HUB) and Hydrocarbon Utilizing Fungi (HUF) were monitored and evaluated throughout the experimental period. Please read full article - http://www.journalajarr.com/index.php/AJARR/article/view/30215 [if !supportLineBreakNewLine] [endif]