A Study of Isolation and Identification of Multidrug Resistant Pseudomonas aeruginosa from Wound ...

Background: In hospitalised patients, Pseudomonas aeruginosa is a clinically significant pathogenic microbe. It is a leading cause of death and morbidity, and it has a variety of mechanisms that render it immune to antibiotics. Given the scarcity of antimicrobial drugs to treat this pathogen's infection, it has become essential to create a new treatment arena for this organism. The number of multidrug-resistant pathogenic strains of Pseudomonas aeruginosa has recently increased. The aim of this study is to isolate and identify multidrug-resistant Pseudomonas aeruginosa from wound specimens, as well as to assess antibiotic-resistant strains of this microbe. Methodology: Between October 2019 and April 2020, 150 clinical wound samples were collected from hospitalised patients at Jinnah Hospital in Lahore. Using the Vitek 2 system and cultural features, morphological characteristics, and various biochemical tests, twenty (20) isolates of Pseudomonas aeruginosa were described. The presence and growth of Pseudomonas aeruginosa were revealed by the development of blue/green, brown/blue, and yellow/green pigments. The percentage of females infected with Pseudomonas aeruginosa was 15%, compared to 11.42 percent in males. The resistance of isolates of Pseudomonas aeruginosa to various antimicrobial drugs was then checked. The most effective antibiotics against Pseudomonas aeruginosa were piperacillin/tazobactam and meropenem. Trimethoprim/sulfamethoxazole showed the highest resistance, with a rate of 75%. Conclusion: The majority of isolates tested positive for four or more substances. Multidrug resistance has emerged as a global problem, as pathogens that cause infections have become increasingly immune to antimicrobial agents.

Please see the link - https://www.journaljpri.com/index.php/JPRI/article/view/30930