A Study of Isolation and Identification of Multidrug Resistant Pseudomonas aeruginosa from Wound

Background: In hospitalised patients, Pseudomonas aeruginosa is a clinically significant pathogenic microbe. It is a leading cause of death and morbidity, and it has a variety of mechanisms that render it immune to antibiotics. Given the scarcity of antimicrobial drugs to treat this pathogen's infection, it has become essential to create a new treatment arena for this organism. The number of multidrug-resistant pathogenic strains of Pseudomonas aeruginosa has recently increased.

The aim of this study is to isolate and identify multidrug-resistant Pseudomonas aeruginosa from wound specimens, as well as to assess antibiotic-resistant strains of this microbe.

Methodology: Between October 2019 and April 2020, 150 clinical wound samples were collected from hospitalised patients at Jinnah Hospital in Lahore. Using the Vitek 2 system and cultural features, morphological characteristics, and various biochemical tests, twenty (20) isolates of Pseudomonas aeruginosa were described. The presence and growth of Pseudomonas aeruginosa were revealed by the development of blue/green, brown/blue, and yellow/green pigments.

Results: Percentage of Pseudomonas aeruginosa in females came out to be 15% as compared to 11.42% in males. This was followed by testing susceptibility of isolates of Pseudomonas aeruginosa to various antimicrobial drugs. Piperacillin/tazobactam and meropenem showed the highest efficacy against Pseudomonas aeruginosa. Highest resistance was exhibited against trimethoprim/sulfamethoxazole which was 75%.

Conclusion: Most isolates showed multidrug resistance to four or more drugs. Development of multidrug resistance has emerged as a global problem with pathogens commonly causing infections becoming increasingly resistant to antimicrobial agents.

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