Alleviated Immunological Activities of Wistar Rat Peritoneal Neutrophils and Macrophages by ......

The aim of this study was to see how polysaccharides from the fruit body of Microporous vernicipes (Polyporales) affected macrophage and neutrophil immunological activities.

Methodology: Polysaccharides were extracted using hot water (PHW) and cool water (PCW), and the behaviours of peritoneal macrophages and neutrophils in response to lipopolysaccharides were investigated. The impact of polysaccharides was investigated. In vitro, vacuole formation, nitroblue tetrazolium dye reduction, lysosomal enzyme activity, nitric oxide, myeloperoxidase in neutrophils, and neutrophil adherence assay were used to evaluate phagocytic activities. Results: Microporus vernicipes cool and hot water polysaccharides dramatically improved macrophage and neutrophil activities, as evidenced by increased vacuole formation and high development of nitric oxide, lysosomal enzyme, and superoxide. The polysaccharides extracts from Microporous vernicipes also induced neutrophils to produce more myeloperoxidase. Neutrophils treated with polysaccharide extracts of Microporous vernicipes adhered to a plastic surface more than those who were not. Polysaccharides from Microporous vernicipes have also been discovered to have a cytotoxic effect at high concentrations, with an IC50 of 2.28. Conclusion: The results of this study show that Microporus vernicipes polysaccharides have immunostimulatory activity by stimulating macrophage and neutrophil activity. Please see the link - https://www.journaljpri.com/index.php/JPRI/article/view/30984