An Overview of Methods of Extraction, Isolation and Characterization of Natural Medicinal Plant ..

Traditional community use of herbal plants is very common, and they contribute to plant biodiversity and conservation.Natural products are important components in complementary and herbal medicine, nutraceuticals, dietary supplements, and pharmaceutical bioactive metabolites of new chemical entities. Due to the diverse flora and fauna biodiversity that provides the requisite chemical diversity, bioactive secondary metabolites from herbal plants of various types are major sources and provide major opportunities for drug active pharmaceuticals. The use of high throughput (HTS) screening in phytochemical research has grown in popularity. programmes looking for a lead

Many phytomedicines for various therapeutic areas have been extracted from herbal products, and phytochemicals of herbal extracts for conventional uses include various forms of bioactive metabolites of pharmaceutical and pharmacotherapeutic nature. The aim of this paper is to provide insight into the extraction, isolation, and characterization of the rich medicinal plant biodiversity of potential pharmaceutical significance, as well as the major drawbacks and challenges in phytochemical extraction, isolation, and characterization in plant extracts.The extraction method, which is a critical stage in the study of bioactive compounds in medicinal plant research, has been studied with a greater focus on phytochemicals in medicinal plants. Following regulatory guidelines and different pharmacopoeia, it's critical to address the benefits and drawbacks of various extraction methods. The study of bioactive molecules in herbal products includes the use of chromatographic techniques such as TLC and HPLC, as well as various phytochemical screeningmethods. In certain cases, non-chromatographic approaches such as Fourier Transform Infrared (FTIR) and immunoassay are used. The difficulties faced by most pharmacy students in data mining of knowledge on phytochemical screening and testing of biological activities in projects involving herbal plants research inspired this paper. This article is also intended to provide students with knowledge on the preclinical drug development process in the hopes of developing a better conventional medicine/ phytomedicine.

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