Antimicrobial Resistance and Virulence Factors of Campylobacter coli Isolated from Chicken in Côte..

The Campylobacters family of bacteria is a prominent cause of gastroenteritis in humans. Consumption of contaminated or cross-infected poultry meat is the main source of illness. The researchers wanted to look at the antimicrobial profile and virulence variables related with Campylobacter coli isolated from the ceaca of chickens slaughtered commercially in Abidjan.

Methodology: A total of 336 chicken ceaca samples were gathered from two Abidjan municipalities' markets and tested using conventional microbiological methods and a PCR-based molecular assay. The isolates' antibiotic sensitivity was assessed using the disc diffusion method, and the presence of virulence genes was investigated using a simple PCR approach.

The results showed that 210/336 (62.50 percent) of the samples tested positive for Campylobacter. For morphological and genotypic study, 53 strains verified as C. coli by PCR detection were used among the isolates. 51/53 of the bacteria tested positive for one or more antibiotic compounds. Nalidixic acid 51/53 (96.22 percent), tetracyclin 49/53 (92.45 percent), and ciprofloxacin 38/53 had the highest rates of antimicrobial resistance (71.69 percent ). Furthermore, MDR including the 3, 4, 5, and 6 antibiotic families was found in 11/53 (20.75%) of isolates. Detection of virulence genes, on the other hand, revealed the presence of the cadF gene in 86.01 percent of isolates, while the three cdt (A, B, and C) genes were found in 82.21 percent.

Conclusion: The presence of high pathogenic Campylobacter coli contamination in the hens investigated was reported in this investigation. The bacteria were identified using molecular techniques, and high resistance to antimicrobials from the fluoroquinolone family was discovered.

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