Augmenting in vitro Shoot Multiplication by Growth Regulators and Photoperiod in Vigna mungo L. (Hep

Through apical meristems and cotyledonary nodal explants, an effective in vitro protocol for mass multiplication of Vigna mungo vars. PU30 and PU31, an important legume crop, was developed. Murashige and Skoog (MS, 1962) medium fortified with 0.5–1.50 mg/L 6-benzyl aminopurine (BA) or Kinetin and 3 percent (w/v) sucrose was used to culture both apical meristems and cotyledonary nodal explants. When the cultures were incubated under continuous light (24 hours), the rate of multiplication was greater than when they were incubated under the 16-hour photoperiod. In the case of var., the average number of different shoots per culture increased from 2.43 to 5.46. Within 4 weeks of culture under a 24-hour photoperiod, PU30 reached 3.12 to 5.82 and var.PU31 reached 3.12 to 5.82. The rate of multiplication increased until the fifth subculture, after which it began to decline. After 2 weeks of culture, the shoots were easily rootable when transferred to half-strength MS basal semisolid medium supplemented with 0.1–1.0 mg/L indole-3-butyric acid (IBA) and 2 percent (w/v) sucrose. Explants had an average of 3.12 to 5.76 roots per explant. In the greenhouse, about 80% of the regenerated plantlets were hardened and successfully established in the soil.

Please see the link :- https://www.journalcjast.com/index.php/CJAST/article/view/31035