Baseline Tryptase Levels Correlate with Baseline Basophil Levels in Chronic Myeloid Leukemia-Chronic

The goal of this study is to see if there is a link between baseline blood basophil count and serum tryptase levels in individuals with chronic phase chronic myeloid leukaemia (CML-CP).

Setting and Design: Based on their baseline BCR-ABL status, 40 newly diagnosed CML-CP patients were included from the Medical Oncology Hematology OPD (done in department of Biochemistry).

Methods and Materials: The level of serum tryptase was determined using a Sandwich ELISA kit, and the number of basophils in the peripheral blood was calculated using an automated cell counter and a peripheral blood film inspection. After converting RNA (extracted from whole blood) to cDNA, BCR-ABL was quantified using real-time PCR. SPSS Version 23 was used for the statistical analysis.

Baseline peripheral blood basophil levels were found to be significantly connected with baseline serum tryptase levels (p0.01), and tryptase level was also correlated with the EUTOS score, which includes basophil count as one of the factors. This could mean that serum tryptase levels in CML-CP can be used as a proxy measure for the basophil compartment.

Conclusions: Serum tryptase can be used as a surrogate marker of the basophil compartment in CML-CP, based on the findings of our investigation and previous studies in the literature.

Please see the link :- https://www.journaljammr.com/index.php/JAMMR/article/view/30731