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Effect of Different Steeping and Malting Regimen on the Amylolytic Activities of Some Improved ....

The effects of different steeping and malting regimens on the production of amylolytic enzymes in three Nigerian improved sorghum varieties were investigated. Steeping consisted of an air rest period followed by a 72-hour steeping regimen. Every 12 hours, samples were taken. The seeds were then germinated for four days before being fired at 50°C for 24 hours. The parameters of grain and malt were investigated. The results revealed that the response of sorghum root length to steep regimen and time varied. After 72 hours of air rests steeping, CSR-02 provided the maximum root length (3.32 cm). CSR-02, Samsorg 44, and Samsorg 14 had germinative energies of 92.00 4.24, 94.00 1.41, and 96.00 1.41 percent, respectively; germinative capacities of 91.00 1.41, 75.50 2.12, and 88.00 2.83; and water sensitivities of 6.50 2.12, 13.50 1.44, and 1.00 0.41. TKW values for CSR-02, Samsorg 44, and Samsorg 14 were 29.73 0.32, 33.85 1.54, and 33.51 0.41 kg, respectively. Variations in sorghum varieties' responses to different steep regime and steep time conditions were also observed. At both steeping regimens, 48 hours seems to be the optimal period for the production of amylolytic activity in all sorghum varieties. Total amylase activity was highest in Samsorg 14 (355.44 g glucose equivalents), followed by Samsorg 44 (278.08 g glucose equivalents). Samsorg 14 had the most -amylase growth (276.93 g glucose equivalents), as well. In all of the sorghum varieties, air rest was found to have a greater impact on -amylase growth.


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