Gene Validation for Brown Plant Hopper Resistance in Rice Varieties Using SSR Markers

The Brown Plant Hopper (BPH), Nilaparvata lugens (Homoptera: Delphacidae) is individual of the most hurtful insect pests inducing significant yield misfortune in most of the edible grain cultivars of Asia. Marker-assisted backcrossing is used to introgress the genes for BPH resistance at which point the selection of benefactor parents to confer sturdy resistance and confirmation of the presence of genes is a prerequisite. Jyothi ultimate popular, widely cultivated edible grain variety in Kerala is secluded along with four fatherly lines viz., PTB33 (Variety released from RARS, Pattambi), IRRI introgressed lines- IR65482-7-216-1-2, IR7103-121-15-B, and a training line RP2068-13-5-8 for validating the occupancy of genes Bph3, BPH18, Bph20, and Bph21 using SSR markers. Parental variety was shown for flags specific to Bph3, BPH18, and Bph20. Besides this, cluster study using the UPGMA method caused 4 clusters. The variety ‘PTB33’ was got in a separate cluster that can be attributed to allure resistant figure against BPH and can be considered as a potential backer. The IRRI introgressed lines- IR65482-7-216-1-2, IR7103-121-15-B, and the breeding line RP2068-13-5-8 can still be well utilized as contributors by proper standardized BPH hide tests for confirming the phenotypic verbalization of BPH resistance.

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