Glycine and Selenium (Separately and in Combination) Reduced Bromate-Mediated Oxidative Stress .....

Bromate toxicity is related to DNA damage and changes in carbohydrate pathways, which can lead to lipid peroxidation and oxidative stress. Glycine and selenium were tested for their effects on bromate-induced alterations in U937 cells and U937-derived macrophages. U937 cells were incubated with or without glycine, selenium, or both before being exposed to bromate in the first experiment. MTT and DCHF-DA assays were used to determine cell viability and ROS development. U937 cells were converted into macrophages using phorbol 12-myristate 13-acetate before being incubated with or without glycine and selenium before being exposed to bromate in the other experiment. Nitric oxide and cytokines (tumour necrosis factor-alpha, interleukin 1 and interleukin 6) secretion were also measured later. Superoxide dismutase and catalase production were also assessed. Bromate induced substantial cytotoxicity and ROS formation, which was decreased when cells were pre-incubated with glycine and selenium, according to the findings (both separately and in combination). Bromate also increased nitric oxide and cytokine secretion in macrophages, which was inhibited by glycine and selenium. Bromate also inhibited the development of superoxide dismutase and catalase, which was reversed to near-control levels by glycine and selenium (both separately and in combination). The antioxidant properties of glycine and selenium are believed to be responsible for their impact. The findings' implications are explored.

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