Histological Effect of Different Inhalants on the Heart and Lungs of Wistar Rats | Asian Journal ...

The respiratory and circulatory systems are the primary targets of most inhalation methods of animal sacrifice. Chemical inhalation methods of animal death for studies involving the heart and lungs are likely to interfere with the research's conclusion. The purpose of this study was to see how the cytoarchitecture of the heart and lungs of Wistar rats was affected by inhaling chloroform, diethyl ether, and formalin as ways of animal sacrifice. Twenty mature male rats were divided into four groups of five for a two-week acclimatisation period. The rats were sacrificed by cervical dislocation (control), chloroform inhalation, diethyl ether inhalation, and formalin inhalation, respectively, after acclimation. The rats' hearts and lungs were removed and fixed in 10% formal saline for histological examinations using the haematoxylin and eosin procedure. The histology of the rats' hearts was not affected by any of the chemical substances breathed, according to the findings. The chemicals inhaled, on the other hand, had negative effects on lung histology, including tissue and cellular necrosis, inflammation, and cytoarchitectural deformation of lung tissues. As a result, we may infer that inhalation sacrifice procedures are not suited for research on the lungs, but they can be employed on the heart, based on the findings of this study. Please see the link :- https://www.journalijrrc.com/index.php/AJRCD/article/view/30110