Identification of Fungal Isolates from Soils of Rice and Napier Grass | Journal of Experimental Agri

The microbial diversity of cultivated soil is a critical resource for maintaining the agricultural and natural ecosystem's functioning capacity. The goal of the study was to determine the prevalence of soil fungi in rice and napier grass fields. At the University farm, soil samples were taken from rice fields that had been flooded and napier grass that had been grown in an upland state. The serial dilution approach was used to cultivate soil fungus on RB media. A total of 13 isolates were cultured, with 11 coming from soil taken from a rice-growing area and two from a napier grass field. Seven out of 11 isolates collected from rice and 2 from napier grass were selected and identified through the morphological characters such as colony color, mycelium diameter, color and segmentaiton, spore size and color. Mycelium growths were examined on the PDA and RB media. Fungal isolates collected in this study were morphologically identified/ characterized as Aspergillus niger, A. flavus, A. terreus, A. carbonarius, A. nidulans, Alternaria sp., Penicillium and Talaromyces. Fungal species differed among the soils were discussed.

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