Identification of SSR Markers Linked to Regions Associated with Protein Content in F2 Population ...

Rice protein improvement is critical for high nutritional quality in rice breeding. The goal of this work was to find protein-associated chromosomal regions in the F2 population of a cross between Swarna and Mahamaya. A parental polymorphism survey between Mahamaya and Swarna was conducted using 24 SSR markers. 4 (16%) of the 24 markers were polymorphic, and 185 F2 plants were individually tested for protein content and genotyping. In the F2 group, total grain protein content ranged from 4.12 to 12.0%. The F2 plants were used to test the selective genotyping strategy. The findings revealed that the three markers RM1369 on chromosome 6, RM263 on chromosome 2, and RM337 on chromosome 8 were unrelated to one another. Because mapping necessitates a minimum of two markers per locus, the data for this investigation were analysed using Recombination Frequency. Recombination frequency values revealed an association between markers RM1369 (Chromosome-6) and high protein content (Recombination frequency=0.41) and low protein content (Recombination frequency=0.25), RM263 (Chromosome-2) and high protein content (Recombination frequency =0.37 percent) and low protein content (Recombination frequency =0.33 percent), and RM337 (Chromosome-8) with high protein content (Recombination frequency = The above markers can be used to identify locations related with rice protein content, according to this trial investigation.

Please see the link :- https://www.journalcjast.com/index.php/CJAST/article/view/31085