In silico Identification and Analysis of Iron (Fe) Transporters in Various Plant Species | Current

Iron is an important micronutrient in plants, as it is required for a variety of functions such as photosynthesis, respiration, and maintaining a balanced redox potential. In human nutrition, iron plays a crucial function. As a result, raising the Fe content of economically important sections by traditional, molecular, and/or transgenic breeding will have a significant impact on human health. As a result, the goal of this study was to find and characterise OsIRT1, OsIRT2, OsVIT1, OsYSL2, OsYSL15, and OsYSL18 homologues in 21 distinct plant species. A total of 51 putative Fe transporter proteins homologues were discovered, each with a sequence length of 555 to 770 amino acids, a molecular weight of 61 to 84.8 kDa, and a pI of 8.2 to 9.4 with a basic nature of 9 to 15 TMDs with an average of 13 TMDs. The plasma membrane was hypothesised to be the subcellular localization of putative Fe transporters, and all of the discovered Fe homologues featured oligopeptide transporter (OPT) domain proteins, which are important in Fe transport and homeostasis. In addition, the research revealed highly conserved residues in probable Fe sequences that might be exploited as potential motif signatures in the search for a new Fe transporter. The interactome investigation of the oryza sativa OsYSL15 transporter revealed a probable interaction with NAS2, NAS1, DMAS1, OsJ 32857, IRT1, IRO2, IDEF1, IRT2, IDI2, all of which are directly engaged in Fe transfer from roots to grain. This research elucidates important theoretical knowledge about Fe genes and protein characteristics, as well as assisting in the molecular manipulation of Fe transporters in various plants for the development of high Fe in the economically important region.

Please see the link :- https://www.journalcjast.com/index.php/CJAST/article/view/31091