In vivo Antistaphylococcal Activity Evaluation of Ocimum gratissimum Linn. (Lamiaceae) Ophthalmic Oi

The purpose of this analysis is to determine the ophthalmic ointment antistaphylococcal potential obtained from the leaves of Ocimum gratissimum. Cutaneous and ocular methanolic extract tolerances of O. Gratissimum leaves is assessed in compliance with the 404 and 405 Guidelines of the Organisation for Economic Co-operation and Development (OECD). 0.3 percent of the Gentamicin ophthalmic ointment was used as an antibiotic guide. Phytomedicine was tested in vivo for antibacterial activity in rabbits infected with S. CIP 4.83 for aureus. Three classes of six animals were treated for 15 days as follows: excipient (vaseline + liquid paraffin), gentamicin ointment and ophthalmic ointment (Ocimum gratissimum) (OGO). 0, 5% Strong methanolic extract cutaneous and ocular tolerances have been observed. The monitoring of microbial contamination carried out on the 5th and 240th days showed that the formulations prepared were compliant with the preparation of cleanliness requirements for topical application to the European Pharmacopoeia. Injection subconjunctival of S. Mucopurulent acute keratoconjunctivitis caused by aureus suspension with edema, and often with eyelid erythema. Therefore, it is on these ocular results that the therapeutic effectiveness of phytomedicine has been measured. Leucoma, eyelid swelling and conjunctiva hyperemia have been developed in treated rabbits without an anti-infective agent. There was only one case of panophthalmitis observed. We observed substantial and progressive reversal of ocular damage in animals treated with gentamicin from the 1st to the 5th days. The eyes were regular on the 15th day; the rabbits had no corneal lesions. A similar pattern is experienced by the batch treated with OGO. The OGO's in vivo therapeutic efficacy was equivalent to that of gentamicin.

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