Investigating the Modulatory Effect of Methanol Extract of Daniellia oliveri (ROLFE) Leaves on .....

Background: The opening of the mitochondrial membrane permeability transition (mPT) pore initiates mitochondrial-mediated cell death, and medicinal plants produce phytochemicals that modulate the mPT pore.

The modulatory effects of crude methanol extract of Daniellia oliveri leaves (CMDO) on the mPT pore were investigated in vitro.

Methods and Design of the Study: Normal protocols were used to test the phytochemical screening and antioxidant activities of crude methanol extract of Daniellia oliveri leaves (CMDO). Vacuum liquid chromatography was used to classify CMDO into three fractions: chloroform fraction (CFDO), ethyl acetate fraction (EFDO), and methanol fraction (MFDO) (VLC). Spectrophotometry was used to test the effects of CMDO, CFDO, EFDO, and MFDO on the mPT pore. Spectrophotometry was used to determine the effects of the most potent fraction on mitochondrial ATPase, Fe-induced lipid peroxidation, and cytochrome c release. The bioactive compounds present in CMDO were identified using GC-MS analysis.

The total flavonoid content (0.4830.02 QE mg/100g), total phenolic content (0.8860.12 GAE mg/100g), total antioxidant potential (0.0390.001 AE mg/100 g), ferric antioxidant reducing ability (IC50=350 g/ml), and 2, 2-diphenyl-1 picrylhydrazyl (DPPH) radical scavenging activity (IC50=166 g/ml) of CMDO were all significant. In the absence and presence of calcium, the maximum induction of mPT pore opening was as follows: CMDO (10.11 folds, 5.18 folds), CFDO (19.9 folds, 16.3 folds), EFDO (7.5 folds, 23.2 folds), MFDO (7.5 folds, 23.2 folds) (22.2 folds, 31.3 folds). The most potent mPT pore-opening fraction (MFDO) increased mitochondrial ATPase activity, inhibited Fe-induced lipid peroxidation, and triggered the release of cytochrome c. Bioactive compounds such as methyl propanamide, Dibutyl phthalate, saturated and unsaturated fatty acids were discovered in CMDO after GC-MS research.

Conclusion: The methanol fraction (MFDO) of CMDO mediated mPT pore opening most effectively by increasing mitochondrial ATPase activity, as evidenced by the release of cytochrome c. (in vitro). This includes MFDO as a potential pharmacologic treatment for diseases caused by a lack of apoptosis.

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