Molecular Characterization of blaCTX-M, blaTEM and blaSHV Beta Lactamases Produced by Uropathogens..

Background: The purpose of this prospective investigation was to determine the frequency with which ESBL-producing bacteria were isolated from urine samples and to investigate their susceptibility patterns. Polymerase chain reaction (PCR) was used to identify the ESBL genes responsible for their resistance (PCR).

The research took place over the course of a year, from January to December of 2016. Patients' urine was processed according to conventional procedures. The disc diffusion method was used to assess antimicrobial susceptibility, according to CLSI standards from 2016.

The cefotaxime, ceftazidime, and double disc diffusion test methods were used to screen the urine isolates for ESBL. For the blaCTX-M, blaTEM, and blaSHV genes, polymerase chain reaction (PCR) was used to identify the ESBL genes that were responsible for their resistance.

The most prevalent organism identified was Escherichia coli (E. coli), followed by Klebsiella pneumoniae, with an ESBL-producing uropathogen prevalence of 20.47 percent. Against urine isolates, the antibiotics Nitrofurantoin and Imipenem were the most effective. The blaCTX-M gene was the most common cause of ESBL production (71.42 percent ).

Conclusion: A considerable number of ESBL-producing strains are causing serious treatment difficulties. To avoid treatment failures in patients with Urinary Tract Infections, ESBL production must be monitored, antibiotics must be used wisely, and infection control measures must be taken (UTI).

Please see the link :- https://www.journaljammr.com/index.php/JAMMR/article/view/30721