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Molecular Marker and Test Cross Information Aid Selective Advancement of F4 Generation of CB174R/Azu

The present inspection was aimed to expand restorer lines for three-line hybrid edible grain using indica/hot japonica derivatives to exploit the bury sub-specific heterosis. From 75 F4 offspring of CB174R/Azucena, two plants were randomly picked and screened utilizing simple sequence repeat gravestones DRRM-RF 3 -10 for Rf3 gene and RM6100 for Rf4 deoxyribonucleic acid. One hundred and five plants possessing either or two together of the genes were test crossed with CMS line COMS 23A. In 67 hybrids judged, the mean pollen potency ranged from 97.3% (CB174R/Azucena 177-4-9) to 13.7% (CB174R/Azucena 13-2-4). The frequency of restorers was extreme (49.25%) followed by prejudiced restorers (29.85%) and partial maintainers (20.90%). The excerpt efficiency for DRRM- RF3-10 and RM6100 markers were 75.75% and 54.54% individually. Segregation for fertility caretaker of collection genes and pollen virility among individual plants within a offspring was witnessed from microscopic and phenotypic data. Based on phenotypic and flag information, it was concluded to advance 53.3% of plants to F5 production to isolate constant restorer lines that maybe exploited in future to produce very heterotic three-line hybrids in rice.


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