Moringa oleifera Leaf Extract Extends Lifespan and Ameliorate HAART Drug-Induced Locomotor..........

The aim of this study was to see how long Moringa oleifera leaf (MOL) extract could protect Drosophila melanogaster from some of the side effects of HAART drugs.

Materials and Methods: Between August 2019 and March 2020, the study was carried out at the Drosophila laboratory of the Africa Centre of Excellence in Phytomedicine Research and Development (ACEPRD), University of Jos, Nigeria. To assess durability, D. melanogaster (1-3 day) were first exposed for life to different concentrations of MOL (50–500 mg), 25 mM Ascorbic acid, or 1000L distilled water. Second, in five replicates, flies were fed 46.56 mg of HAART drugs (Efavirenz- or Dolutegravir-based) alone or with MOL 250 mg or 500 mg per 10 g fly food for seven days. The durability, fecundity, and negative geotaxis of the animals were then assessed. Using whole fly homogenate, the activities of Superoxide dismutase, Catalase, and Malondialdehyde content as biomarkers of oxidative stress were also assessed. P0.05 or (P0.006) (Bonferroni modified P-value for multiple curve comparisons) is used to assess statistical significance.

In contrast to the control groups, the MOL extract improved fly longevity substantially (P0.001). Similarly, supplementation with 500 mg MOL extracts significantly (P0.05) improved HAART drug-induced deficits in climbing ability, fecundity, SOD, and CAT behaviours, as well as MDA content, when compared to groups that were only given HAART drugs.

Conclusion: The findings indicate that M. oleifera leaf extract has antioxidant properties that help to prolong lifespan and minimise HAART drug-induced toxicity. Improved locomotor and reproductive decline, as well as restoration of oxidative stress biomarkers (SOD, CAT, and MDA) in D. melanogaster, all supported this hypothesis.

Please see the link :- https://www.journaljocamr.com/index.php/JOCAMR/article/view/30194