Safety Evaluation of Fermotein: Allergenicity, Mycotoxin Production, Biochemical Analyses and ...

Single-cell proteins (SCPs) are seen as creative and environmentally friendly alternatives to animal-based products. Fermotein is a novel SCP derived from the filamentous fungus Rhizomucor pusillus fermentation. This fungus' toxicity, ability to generate secondary metabolites, and allergenic potential have never been investigated before. There isn't enough information on this species, like other filamentous fungi, to determine whether it's safe to eat. The aim of this study was to look into the protection of Fermotein and its source Rhizomucor pusillus in terms of toxicity, secondary metabolite production, and allergenicity. In addition, there is the possibility of contamination. Methodology: The genome of Rhizomucor pusillus was sequenced and annotated to look for common mycotoxins, antibiotic synthesis pathways, mucormycosis-related virulence factors, and possible cross-reactivity with known food allergens in silico. Laboratory testing confirmed the existence of mycotoxins and allergens. It was also calculated the levels of RNA, heavy metals, and microbiological pollutants. Mycotoxin-related genes were not present in the genome of Rhizomucor pusillus, and no mycotoxins were found in Fermotein. Fermotein contains six proteins that have high homology with five recognised food allergens. There were no gene clusters that corresponded to antibiotic synthesis pathways. Despite the fact that 10 proteins in the Rhizomucor pusillus genome may represent mucormycosis-related virulence factors, no cases of mucormycosis have been identified after oral consumption. Heavy metals and microbiological pollutants were found to be below legal limits, while RNA content was 4.9 0.2 percent of dry matter. Conclusion: Fermotein and its source Rhizomucor pusillus pose no safety risks, with the exception of the possibility of cross-reactivity with five recognised food allergens. This should be considered when communicating with customers. The findings of this study add to the body of evidence used to determine Rhizomucor pusillus' Qualified Presumption of Safety status. Please see the link - https://www.journalejnfs.com/index.php/EJNFS/article/view/30311