Screening of Laccase Producing Fungi Using Agro-Wastes under Different Cultural Conditions | ....

The ability of fungal isolates to produce laccase was examined in this investigation. Laccase production was determined in fungi cultures using a plate test and submerged fermentation with 2' 2' azinobis-(-3-ethyl benzthiazoline -6- suphonate) (ABTS). After 2-4 days of incubation, the plate assay revealed the creation of a green halo due to the oxidation of 2' 2' azinobis-(-3-ethyl benzthiazoline -6- suphonate) (ABTS) produced by lignolytic enzymes. Laccase production was assessed using chosen agro-waste residues (sawdust, plantain, and banana peels). The cultural properties of fungi isolates were used to identify them. Standard mycology methods were used to identify fungal isolates based on their cultural features. On cultures, light microscopy was used to identify fungal isolates based on their morphological and colonial properties, which were then confirmed using a fungal atlas. The Bradford method was also used to determine total protein content. Five of the twelve isolates tested positive for oxidation of 2' 2' azinobis-(-3-ethyl benzthiazoline -6- suphonate) (ABTS), indicating laccase enzyme activity, and were identified as Geotrichum spp., Cephalosporium spp., Trichoderma spp., and Trichoderma spp. Fusarium spp. and Trametes spp. Trichoderma spp. exhibited the highest enzyme activity (57.1 U/l), Trametes spp. had 51.99 U/l, and Fusarium spp. had 29.2 U/l. At 28.04 U/l and 9.72 U/l, respectively, Geotrichum spp and Cephalosporium spp had the lowest concentrations. Further research was conducted using Trichoderma spp. Laccase production was studied in relation to carbon sources, inoculum size, pH, total protein, and shaker speed. Sawdust yielded the most laccase enzyme (151.17 U/l), followed by plantain peel (62.49 U/l), and banana peel (54.94 U/l). The protein concentration in sawdust medium was 45.8 mg/ml. At the sixth day of the fermentation procedure, plantain peel medium had 29.2 mg/ml, while banana peel had 16.8 mg/ml. Sawdust, banana peel, and plantain peel all had optimal pH values of 5.95, 5.94, and 5.83, respectively. Laccase yield of (310.45U/l) was obtained using a shaker incubator (150 rpm) using sawdust as the carbon source at pH 6, temperature 25°C, and inoculum size of 105 spores per ml. As a result, sawdust may be unequivocally declared to be safe and inexpensive, and it could be offered as a potential source of increased laccase enzyme production in a variety of businesses.

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