The Investigation of Antimutagenic Effects on Chromosomes and Cell Division Mechanisms against Mitom

Using chromosomal aberration (CA), micronucleus (MN), and mitotic index (MI) assays, we determined whether liquid extracts of the above-ground portions of Vaccinium myrtillus L. and Rubus idaeus L. exhibit antimutagenic properties against mitomycin C in human peripheral cells.

Methodology: Blood samples of the negative control group (Group I) were allowed to react in a cell culture medium without any treatment, while positive control group (Group II) was allowed to interact in a cell culture medium supplemented with mitomycin-C (MMC) at a dose of 0.3 μL /mL for each chromosome medium. Blood samples of the other groups were allowed to react for 24 hours with 0.3 μL /mL MMC and V. myrtillus L. (Group III, IV, V and VI), and R. idaeus L. (VII, VIII, IX and X) extracts in 0.2 μL /mL, 0.4 μL /mL, 0.8 μL /mL and 1.6 μL /mL doses for each of cell cultures. At the end of the incubation period, culture cells were evaluated by chromosomal aberrations, mitotic, micronucleus and nuclear division index tests.

Results: When chromosome abnormalities and micronucleus indexes were decreased in extract groups, mitotic, nuclear division, and nuclear cytotoxic cleavage indices were raised in comparison to Group II. When comparing extract groups to group II, we found that extract groups had fewer chromosome abnormalities and a lower micronucleus index, whereas mitotic, nuclear division, and nuclear cytotoxic cleavage indices were higher.

At the working doses, aqueous extracts of V. myrtillus L. and Rubus idaeus L. were found to have considerable antimutagenic effects on human peripheral lymphocyte cells.

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