Partial Purification of a Catalase from an Improved Nigerian Sorghum Grain Variety | Asian Journal o

Also known as basal cell nevomatosis (CBN), Gorlin-Goltz syndrome is a rare hereditary condition that belongs to the family of neurocristopathies or diseases caused by neural ridge defects. Four patients suffering from this syndrome have been followed up in the stomatology and maxillofacial surgery department in Casablanca, including the family type in two cases: mother and son, and two isolated cases, followed up and treated in the maxillofacial surgery department of the hospital in Casablanca on 20 August. A clinical, biological and radiological evaluation was carried out, accompanied by surgical care of the 3 patients, anatomical and pathological review and a strict monitoring plan. Catalases are key components of cellular detoxification pathways which, by catalyzing the decomposition of hydrogen peroxide into water and molecular oxygen, prevent highly reactive hydroxyl radicals from forming. Their presence in brewery grains prevents large brewery enzymes from being inactivated and also avoids lipid peroxidation. Crude catalase was obtained from a sorghum grain variety to determine its occurrence and to create some of its properties in sorghum, which has become comparable to barley as an essential brewing grain. Preliminary purification of catalase from the sorghum grain variety used, NRL-3, showed that the enzyme, with a final specific activity of 32 Umg-1 proteins, was purified 3.2 times from the crude protein to give a 49 percent yield of the partially purified enzyme. A positive indication of the presence of sorghum catalase on SDS PAGE with positive bands occurring between the 48-62 kDa range was also found. Therefore, sorghum catalase's molecular weight most likely falls between the two bands. The enzyme displayed a small pH range occurring at pH 7 with optimum activity. Similarly, at 40 ° C, its optimum operation temperature took place. The first recorded attempt to purify catalase from sorghum is this work.

Please see the link :- https://www.journalajocs.com/index.php/AJOCS/article/view/19051